| Serie 1 V.V. +1.8 |
| Fernanda Gonzalez Gomez | Atlético Parral | 3 | 26.95 (CR) | 1 q |
| Fernanda Isabel Bustos Saravia | DSC Diamantes de Coronel | 4 | 27.95 | 3 q |
| Antonia Inés Inda Crisóstomo | Atlético Quilamapu | 5 | 28.91 | 8 q |
| Amanda Alejandra Sanhueza Jiménez | Atlético Olimpo Chiguayante | 6 | 29.15 | 10 |
| Anais Antonella Ortiz Bouldres | Deportivo Atlético Coronel | 8 | 29.50 | 14 |
| Camila Antonia Muñoz Robles | Atletismo Juan Silva - Lota | 2 | 29.88 | 15 |
| Alejandra Mellado Cuevas | Atlético Chiguay Antu | 7 | 30.31 | 16 |
| Camila Fernanda Jiménez Gutiérrez | Atletismo Penco | 1 | 36.91 | 19 |
| Serie 2 V.V. +1.1 |
| Narel Pascal Rivas San Martín | DSC Diamantes de Coronel | 3 | 27.76 | 2 q |
| Maria Jesus Lara Salazar | Atletismo Cauquenes | 4 | 28.14 | 4 q |
| Xiomara Antonia Herrera Crisosto | CADECU | 5 | 29.32 | 12 |
| Maria Elena Aguilera Gonzalez | Coronel Masters | 2 | 29.42 | 13 |
| Josefa Valdebenito Toledo | Atlético Los Ángeles | 1 | 30.52 | 17 |
| Simona Andrea Cambiaso Pérez | Atlético Los Ángeles | 6 | DNS | |
| Cintia Aylen Gonzalez Villegas | Atlético San Pedro | 7 | DNS | |
| Serie 3 V.V. -0.5 |
| Ema Antonia Parra Muñoz | SSCC Concepción | 5 | 28.19 | 5 q |
| Cecilia Antonia Lara Salazar | Atletismo Cauquenes | 4 | 28.36 | 6 q |
| Ayrin Zolanyel Rosales Fanay | Atlético Aves | 3 | 28.84 | 7 q |
| Mar Aniela Reyes Bastidas | Atlético Olimpo Chiguayante | 1 | 29.14 | 9 |
| Constanza Andrea Reyes Manríquez | Atletismo Juan Silva - Lota | 6 | 29.21 | 11 |
| Vannia Elizabeth Monardes Molina | Atlético Aves | 7 | 32.99 | 18 |
| Florencia Paz Alday Flores | Deportivo Atlético Coronel | 2 | DNS | |
