| Serie 1 V.V. +0.0 |
| Fernanda Gonzalez Gomez | Atlético Parral | 3 | 26.48 | 1 q |
| Maria Jose Moscoso Romero | Atlético Aves | 4 | 27.99 | 10 q |
| Fernanda Isabel Bustos Saravia | DSC Diamantes de Coronel | 6 | 28.03 | 11 |
| Catalina Alejandra Becerra Sepúlveda | Atlético Quilamapu | 2 | 28.79 | 12 |
| Narel Pascal Rivas San Martín | DSC Diamantes de Coronel | 5 | 28.86 | 13 |
| Constanza Andrea Reyes Manríquez | Atletismo Juan Silva - Lota | 7 | 29.16 | 14 |
| Camila Javiera Muñoz Muñoz | Atlético Nahuén | 1 | 31.06 | 19 |
| Serie 2 V.V. +1.2 |
| Valentina Valladares Escobar | Atlético Nahuén | 6 | 26.82 | 2 q |
| Mayte Josefa Sepúlveda Vergara | Atlético Quilamapu | 3 | 27.35 | 3 q |
| Francisca Madeleine Valderrama Nuñez | Atletismo Penco | 4 | 27.56 | 7 q |
| Josefa Helena King Martínez | Atlético Olimpo Chiguayante | 5 | 27.93 | 9 |
| Camila Antonia Muñoz Robles | Atletismo Juan Silva - Lota | 7 | 29.21 | 15 |
| Ignacia Ortiz Palma | Atlético Olimpo Chiguayante | 2 | 29.63 | 17 |
| María Jose Fuentes Silva | Coronel Masters | 1 | DNS | |
| Serie 3 V.V. +0.5 |
| Angelica Alejandra Reinoso Godoy | Deportivo Atlético Coronel | 6 | 27.39 | 4 q |
| Anais Belen Bustos Campos | Atletismo Penco | 4 | 27.50 | 5 q |
| Josefa Emilia Plaza Friz | Atlético Los Ángeles | 3 | 27.53 | 6 q |
| Ayrin Zolanyel Rosales Fanay | Atlético Aves | 5 | 27.74 | 8 q |
| Maria Elena Aguilera Gonzalez | Coronel Masters | 1 | 29.62 | 16 |
| Simona Andrea Cambiaso Pérez | Atlético Los Ángeles | 2 | 30.12 | 18 |
| Florencia Paz Alday Flores | Deportivo Atlético Coronel | 7 | DNS | |
