| Serie 1 V.V. +0.0 |
| Constanza Sepulveda Fuentealba | Oassi | 5 | 26.82 | 1 q |
| Anais Belen Bustos Campos | Atletismo Penco | 1 | 27.47 | 2 q |
| Constanza Fernanda Plaza Martinez | Atlético Portalesfit | 6 | 28.10 | 3 q |
| Aileen Noemi Bustos Campos | Atletismo Penco | 2 | 28.49 | 4 q |
| Nicole Pavez Morales | Atlético Santiago | 8 | 29.09 | 5 q |
| Mayte Josefa Sepúlveda Vergara | Atlético Quilamapu | 3 | DNS | |
| Andrea Loreto González Aránguiz | Deportivo Atlético Armada | 4 | DNS | |
| Martina Torreblanca Palacios | Atlético Rauten | 7 | DNS | |
| Serie 2 V.V. -2.1 |
| Magdalena Rosenfeld Ramírez | Atlético Santiago | 4 | 26.54 | 1 q |
| Laura Isabella Tilleria Vargas | Atlético Santiago | 5 | 27.09 | 2 q |
| Estefany Pavez Morales | Atlético Santiago | 6 | 27.36 | 3 q |
| Antonella Simonetti Gebhard | Manquehue | 3 | 27.84 | 4 q |
| Annahy Morales Mira | Atlético Portalesfit | 7 | 28.51 | 5 q |
| Lorenza Canles Espinoza | Atlético Santiago | 8 | 31.68 | 6 q |
| Carla Francisca Padilla Azocar | Atlético Harambeé | 1 | DNS | |
| Josefa Antonia López Jeria | Nicolás Carvajal - Talagante | 2 | DNS | |
| Serie 3 |
| Lissette Fernanda Jara Gonzalez | Nicolás Carvajal - Talagante | 2 | DNS | |
| Aileen Victoria Villanueva Videla | Deportivo Atlético Armada | 3 | DNS | |
| Fabiola Gina Olga Viano Pino | Atlético Limache | 5 | DNS | |
| Daniela Paz Montané Paublo | Atlético Valparaiso | 7 | DNS | |
| Dafne Anais Reyes Muñoz | Atlético Estación Central | 8 | DNS | |
